2. In order to perform a PCR reaction you need to start with template DNA and have a thermocycler (PCR machine). According to table 6.2 on pg 179 of your textbook what are 4 other components that you need in order to perform a successful PCR reaction?
1. What technique do you use to be able to visualize and detect a PCR product?
2. In order to perform a PCR reaction you need to start with template DNA and have a thermocycler (PCR machine). According to table 6.2 on pg 179 of your textbook what are 4 other components that you need in order to perform a successful PCR reaction?
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1. What are the controls for this lab that ensure your results are correct?
2. Do you think your test food is a GMO? 1. For this GMO lab we will be using a 3% TAE agarose gel for our electrophoresis, rather than a 1% TAE agarose gel. Think about what our product is for this lab. Why would we need a 3% gel and not a 1% gel?
1. Next week you will be testing food to see if it is genetically modified. You will be grinding up a known non-GMO and comparing its bands to an undetermined test food. If you had to use the same mortar and pestle for both, which one should you grind up first to prevent contamination, the non-GMO or the test food?
2. Foods containing GM crops are highly processed. How might the DNA obtained from processed products vs whole plants differ? |
Mrs. HusselsteinBiotechnology II Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom. Archives
December 2018
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