2. If you don't get growth on the LB/AMP/ KAN plate, what could you do to encourage growth?
1. If you do get growth on the LB/AMP/ KAN plate, what test or procedure could you do to verify that your growth is due to a successful transformation?
2. If you don't get growth on the LB/AMP/ KAN plate, what could you do to encourage growth?
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No warm-up, please go to your lab stations. We will begin lab immediately.
1. Why will you need to cool the agar, before adding antibiotics?
2. What types of plates do you need to prepare for the bacterial transformation? 1. how will you inactivate your restriction enzymes?
2. Why is this step important? 1. Review you materials list for the restriction digest. List 4 items that you need.
1. Why are we streaking plates rather than just spreading bacteria on plates?
2. How will you cool off your loop before getting a bacteria colony? 1. Why is it necessary to use foil when boiling our media? What does it do?
2. Why is it necessary to use a stir bar when making media? 1. List the materials you would need for bacterial transformation.
2. What senior activities are you looking forward to? |
Mrs. HusselsteinBiotechnology II Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom. Archives
December 2018
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