2. How will you detect your PCR product?
1. How will you extract DNA from food next week?
2. How will you detect your PCR product?
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1. What is required from a cell in order to conduct a PCR?
2. What are the 3 stages of PCR? Please list them in the order that they occur. 1. What does DNA polymerase do in PCR?
2. What does PCR do? 1. How was your Thanksgiving break? What was your favorite or least favorite part about it?
1. Calculate the amount of agarose needed to prepare 40 ml of 1% TAE agarose solution?
1. Why do you think we would set the spectrophotometer to a wavelength of 260nm to quantitate DNA?
2. How will a gel electrophoresis detect DNA? 1. When calculating transformation efficiencies, what number did you use as the bottom number?
2. What are the units of this number? State whether the plasmid was in the pellet(p), supernatant(s), bound to column (c), or in the flowthrough(f).
1. If 20g of LB broth powder is required to make 1 L of LB broth, how much LB broth powder is needed to make 50ml of LB broth?
2. How much Lb broth do you need to make for our protein purification lab? |
Mrs. HusselsteinBiotechnology II Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom. Archives
December 2018
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