ECHS Biology & Biotechnology
with Mrs. Husselstein
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Biotechnology II

Warm Ups

Monday, 10/31/2016

10/30/2016

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1.  What will we do in our DNA quantitation lab overall?

2.  What exactly are we measuring?
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Friday, 10/28/2016

10/28/2016

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1.  After centrifuging the supernatant through the column, where is the plasmid?

2.  After centrifuging the elution through the column, where is the plasmid?
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Thursday, 10/27/2016

10/26/2016

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1.  After adding your pGLO broth to the microtubes and spinning them, where was your plasmid? (pellet or supernatant)

2.  After following the miniprep protocol and spinning your microtubes where was your plasmid? (pellet or supernatant)
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Wednesday

10/25/2016

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No warm up, purification of pGLO lab.
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Tuesday, 10/25/2016

10/24/2016

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1.  In our bacterial transformation lab, are our LB/amp plates antibiotic resistant or are our bacteria?  Explain.

2.  What two factors must be present in the bacteria’s environment for you to see the green color? 
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Monday, 10/24/2016

10/23/2016

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1.  What may have happened to our bacteria cells last week to cause them not to fluoresce?

2.  When calculating transformation efficiencies what two things do you need to know?
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Friday, 10/21/2016

10/20/2016

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1.  On which of the plates would you expect to find bacteria most like the original untransformed E. coli colonies you initially observed? Explain your prediction.

2. Which plates should be compared to determine if any genetic transformation has occurred? Why?
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Thursday, 10/20/2016

10/20/2016

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Wednesday, 10/19/2016

10/19/2016

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1.  What are some possible reasons the bacteria did not grow on our last attempt?

2.  Why do we need to grow bacteria in the first place if you have them present in liquid form already?
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Tuesday, 10/18/2016

10/19/2016

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No warm-ups.  Making agar plates: take 2.
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    Mrs. Husselstein

    Biotechnology II Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom.

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