1. What will we do in our DNA quantitation lab overall?
2. What exactly are we measuring?
2. What exactly are we measuring?
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1. After centrifuging the supernatant through the column, where is the plasmid?
2. After centrifuging the elution through the column, where is the plasmid? 1. After adding your pGLO broth to the microtubes and spinning them, where was your plasmid? (pellet or supernatant)
2. After following the miniprep protocol and spinning your microtubes where was your plasmid? (pellet or supernatant) 1. In our bacterial transformation lab, are our LB/amp plates antibiotic resistant or are our bacteria? Explain.
2. What two factors must be present in the bacteria’s environment for you to see the green color? 1. What may have happened to our bacteria cells last week to cause them not to fluoresce?
2. When calculating transformation efficiencies what two things do you need to know? 1. On which of the plates would you expect to find bacteria most like the original untransformed E. coli colonies you initially observed? Explain your prediction.
2. Which plates should be compared to determine if any genetic transformation has occurred? Why? 1. What are some possible reasons the bacteria did not grow on our last attempt?
2. Why do we need to grow bacteria in the first place if you have them present in liquid form already? |
Mrs. HusselsteinBiotechnology II Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom. Archives
February 2018
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