2. When DNA unzips what side is being copied the inside or the outside?
1. Why do you think PCR is often critical in forensics?
2. When DNA unzips what side is being copied the inside or the outside?
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1. Nucleotide triphosphates (dNTPs) are added to the growing DNA strand during the _______________ phase.
2. During the annealing step,...
What is the function of each of the following in a PCR reaction?
1. Heat: 2. Primer: 3. Taq Polymerase: 1. Describe the main components in a PCR reaction tube.
2. What are the three steps involved in the PCR reaction process? 1. Who is credited for inventing the process of PCR and what company was he working for?
2. What does PCR do? Review
1. What is replication? 2. List as many parts/components involved in replication that you can think of. 1. Look up PCR. What does it stand for?
2. Look up. What does polymerase do? How is it different from lactase, or nuclease? State whether the following statements have to do with transformation, transduction, conjugation.
1. Involves naked DNA 2. Genetic material is adsorbed and injected 3. Competent cell are required. 4. A pilus is produced. 5. Requires cell to cell contact. 6. Mediated by a virus. 7. Involves a phage. 8. An F plasmid encodes for attachment proteins. 1. In your text you were told that EDTA is used in a plasmid purification to chelate the divalent cations (magnesium +2 ions). Define chelating.
2. What does a plasmid purification do? ?1. What does electroporation do to a cell?
2. What is another method of introducing DNA into a cell (other than using CaCl2). |
Mrs. HusselsteinBiotechnology II Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom. Archives
December 2018
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