2. A sample of bacteria growing on LB/amp agar is streaked onto a plate containing LB/amp/agar. Will the bacteria grow, glow, or both?
1. Non-transformed bacteria are placed in LB/amp/ara agar. Will the bacteria grow, glow, or both?
2. A sample of bacteria growing on LB/amp agar is streaked onto a plate containing LB/amp/agar. Will the bacteria grow, glow, or both?
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1. What is the purpose of including the ara C gene in our plasmid?
2. What causes the GFP protein to glow under UV light? 1. Why is having an origin or 'ori' important to include in a plasmid?
2. What is the purpose of including a 'bla" gene in our plasmid? 1. 1. Other than making fluorescent bacterial cells, what other products can be made through transformation? Provide two examples.
2. Once bacterial transformation is complete, how do you determine it's success? 1. What do you put into bacteria in order to transform them?
2. List the steps needed to create your answer to #1. 1. What is a possible risk associated with GMO?
2. what is one advantage to them (in general)? 1. When it comes to bacteria, what makes them classified as competent?
2. What percent of bacteria are naturally competent? 1. Let's assume that we figured out how to use the CRISPR gene and control any possible mutations or spreading of it into other populations so that it was safe. Tell me two ways you would use this technology.
1. How do you feel about Doudna and her colleague not receiving the patent for being able to use CRISPR on humans, plants, or animals?
2. How do you feel about CRISPR already being used on human cells? 1. In your opinion, what is the greatest fear regarding GM crops?
2. Do you think this fear is justified or not? Why? |
Mrs. HusselsteinBiotechnology II Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom. Archives
December 2018
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