ECHS Biology & Biotechnology
with Mrs. Husselstein
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Biotechnology II

Warm Ups

Monday, 2/29/2016

2/29/2016

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1.  What is the process of making a protein?  (Hint: Central Dogma)

2.  Where does this process take place in a cell?
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Thursday, 2/25/2016

2/24/2016

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1.  Consider the different steps in the GMO lab, what do you think went wrong in our procedure that prevented us from seeing plant DNA in our gels?  


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Wednesday, 2/23/2016

2/23/2016

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1.  Where did our PCR product come from?

2.  What will the molecular weight ruler help us determine?
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Monday 2/22/2016

2/22/2016

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1.  Why do we need to have a GMO positive DNA?

2.  Why do we need to prepare two PCR reactions on the non-GMO, test food, and GMO+ DNA?
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Tuesday, 2/23/2016

2/22/2016

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1.  What is required from the cell to conduct a PCR?

2.  How can we know we have successfully attained a PCR product?
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Wednesday, 2/16/2016

2/16/2016

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1.  What are we attempting to do today in lab?

2.  Give two reasons why we are using specific foods to do this lab? 
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Monday, 2/8/2016

2/8/2016

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1.  What is the purpose of MgCl in PCR reactions?

2.  What is the purpose of DNA polymerase in PCR reactions?
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Friday, 2/5/2016

2/5/2016

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1.  Describe the importance of temperature (what does it help achieve) during each step of PCR? 
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Wednesday, 2/3/2016

2/3/2016

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1.   List and describe what happens during the 3 steps of PCR.
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Tuesday, 2/2/2016

2/2/2016

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1.  Based on what we have covered so far,  what is the purpose of PCR?  What does it do?

2.  What are some possible applications of PCR? 
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    Mrs. Husselstein

    Biotechnology II Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom.

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