1. What is the process of making a protein? (Hint: Central Dogma)
2. Where does this process take place in a cell?
2. Where does this process take place in a cell?
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1. Consider the different steps in the GMO lab, what do you think went wrong in our procedure that prevented us from seeing plant DNA in our gels?
1. Where did our PCR product come from?
2. What will the molecular weight ruler help us determine? 1. Why do we need to have a GMO positive DNA?
2. Why do we need to prepare two PCR reactions on the non-GMO, test food, and GMO+ DNA? 1. What is required from the cell to conduct a PCR?
2. How can we know we have successfully attained a PCR product? 1. What are we attempting to do today in lab?
2. Give two reasons why we are using specific foods to do this lab? 1. What is the purpose of MgCl in PCR reactions?
2. What is the purpose of DNA polymerase in PCR reactions? 1. Describe the importance of temperature (what does it help achieve) during each step of PCR?
1. List and describe what happens during the 3 steps of PCR.
1. Based on what we have covered so far, what is the purpose of PCR? What does it do?
2. What are some possible applications of PCR? |
Mrs. HusselsteinBiotechnology II Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom. Archives
December 2018
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