2. What is the molucular weight ruler for?
1. Why do you think you were given a GMO positive sample to run? (what was the purpose of this?)
2. What is the molucular weight ruler for?
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1. What will you be adding to your PCR tubes today?
2. What do you think is the purpose of adding this? Welcome back!
Before we went on break you extracted DNA from various samples of food. Today you will be setting up the PCR reactions and running a PCR. 1. Why do you think it's important to recap your tubes immediately after adding each reagent and template DNA? 2. What should you avoid transferring from your tubes containing the template DNA? 1. How will be extracting DNA from GMO and non-GMO samples?
2. How many times will the PCR need to cycle? 1. According to your reading, what were some good sources for obtaining a GMO result?
2. According to your reading, what were some good sources for obtaining a non-GMO result? 1. What is a GMO?
1. We will be detecting whether certain food items are GMO's using PCR. What component(s) will you need that help detect a GMO?
2. What type of samples will you need for comparison? Now that we're done with Bacterial Transformation and Purification. Let's get back to PCR.
1. What is PCR used for? 2. What are some necessary components for PCR to occur? 1. What does a spectrophotometer do?
2. How will it indicate whether plasmid DNA is present? 1. How do your procedures say to compare your sample bands with the standards? Be specific.
2. What was the volume of the plasmid solution that was loaded into the gel for the purified plasmid? For the dilution? |
Mrs. HusselsteinBiotechnology II Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom. Archives
December 2018
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