ECHS Biology & Biotechnology
with Mrs. Husselstein
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Biotechnology II

Warm Ups

Wednesday, 2/1/2017

1/31/2017

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1.  In your Bradford assay, what color change occurred when proteins were present?

2.  What was the protein concentration of your milk samples?
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Tuesday, 1/31/2017

1/30/2017

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1.  What is the purpose of size exclusion chromatography?

2.  What other biotechnology techniques does this remind you of?
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Monday, 1/30/2017

1/29/2017

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1.  What is the purpose of Lowry's and Bradford Assays?

​2.  How does a spectrophotometer help with these assays?
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January 27th, 2017

1/27/2017

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1.  List 4 ways that prokaryotic and eukaryotic processes and protein synthesis differ from each other.
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Thursday, 1/26/2017

1/25/2017

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1.  What group of proteins in mammalian milk does Coomasie blue in the Bradford reagent interact with?

2.  Which type of milk do you predict will have the biggest color change?
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Wednesday, 1/25/2017

1/24/2017

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1.  Why do you think Bradford reagent is considered the best reagent for protein quantitation?

​2.  What test is the least effective?
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Tuesday, 1/24/2017

1/23/2017

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1.  Given the variety of milk and milk substitute options, which one do you prefer to ingest?

2.  What are the 3 processes necessary for eukaryotic protein synthesis?
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Monday, 1/23/2017

1/20/2017

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1.  What are two types of proteins found in milk?

2.  What is the dye in Bradford reagent that causes a change in color?
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Friday, 1/20/2017

1/19/2017

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1.  List 4 functions that proteins perform in biological systems.

2.  The Bradford assay depends on which color shift to indicate the presence of proteins?
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January 18th, 2017

1/18/2017

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1.  What does a protein's function depend on?

2.  What are the monomers of a protein?
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    Mrs. Husselstein

    Biotechnology II Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom.

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