ECHS Biology & Biotechnology
with Mrs. Husselstein
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Biotechnology II

Warm Ups

Friday, 4/28/2017

4/28/2017

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1.  How much broth will you need in order to transform bacteria?

2.  What two things do you need in order to perform a restriction digest?
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thursday, 4/27/2017

4/27/2017

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1.   Briefly describe the bacterial transformation protocol.
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Wednesday, 4/26/2017

4/26/2017

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1.  How will you determine if your transformation was a success? Qualitatively?

2.  Quantitatively?
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Tuesday, 4/25/2017

4/25/2017

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1.  What do you need to have on hand in order to create a new plasmid with two genes with antibiotic resistance?
2.  How will you splice the two plasmids?
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Monday, 4/24/2017

4/24/2017

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1.  What's larger DNA or a protein?

2.  If we needed to run a gel electrophoresis what would we need to change based on the size of the molecule?
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Thursday, 4/13/2017

4/13/2017

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1.  What are you looking forward to this spring break?

2.  What are you looking forward to the rest of this school year?
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Wednesday, 4/12/2017

4/12/2017

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1.  When performing a bacterial transformation what safety procedures should you follow?

2.  What is a bacterial transformation?
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Tuesday, 4/11/2017

4/11/2017

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1.  What do restriction enzymes do?  How do they perform their job?

2.  What molecule will be used to anneal the fragments created by a restriction digest together?
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Monday

4/10/2017

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1.  What does a plasmid map show?

2.  How does a plasmid replicate?
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    Mrs. Husselstein

    Biotechnology II Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom.

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