1. How much broth will you need in order to transform bacteria?
2. What two things do you need in order to perform a restriction digest?
2. What two things do you need in order to perform a restriction digest?
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1. Briefly describe the bacterial transformation protocol.
1. How will you determine if your transformation was a success? Qualitatively?
2. Quantitatively? 1. What do you need to have on hand in order to create a new plasmid with two genes with antibiotic resistance?
2. How will you splice the two plasmids? 1. What's larger DNA or a protein?
2. If we needed to run a gel electrophoresis what would we need to change based on the size of the molecule? 1. What are you looking forward to this spring break?
2. What are you looking forward to the rest of this school year? 1. When performing a bacterial transformation what safety procedures should you follow?
2. What is a bacterial transformation? 1. What do restriction enzymes do? How do they perform their job?
2. What molecule will be used to anneal the fragments created by a restriction digest together? 1. What does a plasmid map show?
2. How does a plasmid replicate? |
Mrs. HusselsteinBiotechnology II Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom. Archives
February 2018
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