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1. On what basis does agarose gel electrophoresis separate molecules?
2. What would happen if distilled water were substituted for TAE buffer in the chamber solution?
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1. Once poured, why should the agarose gel be opaque before removing the comb from the gel casting tray?
2. In DNA electrophoresis, the comb is placed at one end of the gel. For the dye electrophoresis we will be conducting, we placed the comb in the center. Why? (Hint: DNA migrates to one end for a reason) 1. We will be boiling TAE agarose solution in lab today. What precautions do you need to take?
2. What do you need to do after the TAE agarose solution boils? 1. Look up the MSDS for TAE, what is harmful about it?
2. True or False. When diluting a liquid you need to add more solute than solvent. 1. 2.00 L of 0.800 M NaNO3 must be prepared from a solution known to be 1.50 M in concentration. How many mL are required?
The next problem involves slightly more calculation than the discussion above. 1) calculate total moles, 2) calculate total volume, 3) divide moles by volume to get molarity You can also think of it this way: M1V1 + M2V2 = M3V3 Where the '1' refers to one starting solution, '2' to the other starting solution and '3' refers to the mixed solution (hints: V3 is the total volume after mixing and M3 is almost always the unknown) 2. Calculate the final concentration if 2.00 L of 3.00 M NaCl and 4.00 L of 1.50 M NaCl and 4.00 L of water are mixed. Assume there is no volume contraction upon mixing. 1. What do you remember about pipetting?
2. Pick your teams of three, write their name and task. Who will measure out the necessary amount of TAE buffer? Who will measure out the necessary amount of liquid? Who will check for accuracy and make sure the other two are measuring the correct amount? 1. What does it mean to dilute something?
2. What do you recall about the procedure or equation for diluting? 1. Before analyzing DNA using a gel electrophoresis, what do you need to do to the DNA?
2. How do you isolate DNA? 1. Why do we need to isolate DNA?
2. In cloning, why do we use vectors? 1. Go to join.quizizz.com. The game code will be provided to you. Enter your FULL NAME. Answer the questions.
2. Screenshot your final score and enter it as your warm-up answer for today. |
Mrs. HusselsteinBiotechnology II Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom. Archives
February 2018
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