1. What are the three steps in PCR and what are the temps?
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1. Based on yesterday's notes, tell me two enzymes that are used during DNA replication and what their function is.
1. Tell me something you know about PCR, if nothing then say so.
1. What enzyme is responsible for making a copy of a plasmid?
1. If restriction enzymes cut DNA, then what glues it back together?
1. What does a DNA plasmid need to have in order to make a copy of itself? You can refer to a plasmid map for clues.
1. What is a plasmid and where are they found?
2. What do you think a plasmid map might show? 1. How are you? If you'd like, you can tell me about something positive or mildly pleasant that happened this week or you can tell how much this week sucked.
1. What does electrophoresis-TAE buffer (the liquid we put in our gel electrophoresis chambers) contain to help conduct electricity?
2. Tell me one thing you will remember about this DNA fingerprinting unit. |
Mrs. HusselsteinBiotechnology II Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom. Archives
December 2018
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