2. What did you need to add to the cells in order to do this? There are several, name one.
1. A miniprep (aka plasmid purification) purifies plasmids (DNA) away from the rest of the bacterial cells components. You have done a similar procedure in Biology when you took DNA out of strawberry or cheek cells. What was it called?
2. What did you need to add to the cells in order to do this? There are several, name one.
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1. What is a miniprep?
2. Why do you think purification is important to the process of creating recombinant DNA products? 1. What was your favorite or most interesting or easiest lab or activity?
2. What was your least favorite or least interesting or hardest lab or activity? 1. What sugars are bacterial cell walls made of? (pg 61)
2. What type of media is used for long term storage of bacterial cultures? 1. What information do you need to know to calculate the transformation efficiency?
2. Why can't we just use the amount of plasmid we pipetted as the amount of DNA used? 1. Are you ready for senior interviews?
2. What are your strengths? What are your weaknesses? 1. Why do you think it is important to not just write procedures but also read procedures before doing a lab?
2. If you've made your media, what was the tricky or difficult about it? If you haven't made your media, what do you anticipate will be tricky or difficult to do? 1. How much agar will you need to prepare for this lab?
2. How much ampicillin will you need to add? |
Mrs. HusselsteinBiotechnology I Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom. Archives
December 2018
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