2. Complete this rule for the movement of DNA fragments through the agarose gel. “The larger the DNA fragment, the _______________”
1. You've completed your gel electrophoresis and are observing the bands of each fragment. If it were possible to weigh each of the fragments which one would be the heaviest? Why?
2. Complete this rule for the movement of DNA fragments through the agarose gel. “The larger the DNA fragment, the _______________”
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1. Would you want to know if you inherited a gene that could lead to a disorder? Why or why not?
2. Please fill out this form. 1. In this assignment, why does DNA need to be cut with restriction enzymes?
2. Once spliced what can it be used for? A linear DNA molecule is represented below. The DNA is represented by one line, although in actuality DNA has two strands. 1. If the DNA molecule has two restriction sites, A and B, for a specific restriction enzyme, how many fragments would be produced if the DNA is cut by that enzyme?
2. Which fragment would be the largest? 3. Which fragment would be the smallest? 1. Where would the larger fragments, those with a greater number of base pairs, be located, toward the top of the gel or the bottom? Why?
2. Suppose you had five hundred pieces of each of the four fragments, how many bands would there be? 1. Describe the function of restriction enzymes in nature.
2. Describe the function of restriction enzymes in biotechnology. 1. What do you think determines the rate at which fragments migrate through a gel?
2. What would happen if you were to touch the gel while the electrophoresis chamber was running? What must you remember to do in order to keep that from happening? 1. At what voltage did we run our gel at? For how long?
2. Once poured, why should the agarose gel be opaque before removing the comb from the gel casting tray? 1. What would happen if distilled water were substituted for TAE buffer in the chamber solution?
2. In DNA electrophoresis, the comb is placed at one end of the gel. For the dye electrophoresis we will be conducting, we placed the comb in the center. Why? 1. We will be boiling TAE agarose solution in lab today. What precautions do you need to take?
2. What do you need to do after the TAE agarose solution boils? |
Mrs. HusselsteinBiotechnology I Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom. Archives
December 2018
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