1. In your Restriction Digest Lab, which lane in your gel electrophoresis contained the molecular weight standards? What was in it?
2. How did you determine the band sizes of the unknown fragments?
ECHS Biology & Biotechnology with Mrs. Husselstein |
Warm Ups
You'll need to refer to your lab notebook.
1. In your Restriction Digest Lab, which lane in your gel electrophoresis contained the molecular weight standards? What was in it? 2. How did you determine the band sizes of the unknown fragments?
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1. Where would the larger fragments, those with a greater number of base pairs, be located, toward the top of the gel or the bottom? Why?
2. Suppose you had five hundred pieces of each of the four fragments, how many bands would there be? A linear DNA molecule is represented below. The DNA is represented by one line, although in actuality DNA has two strands. 1. If the DNA molecule has two restriction sites, A and B, for a specific restriction enzyme, how many fragments would be produced if the DNA is cut by that enzyme?
2. Which fragment would be the largest? 3. Which fragment would be the smallest? 1. According to your lab manual, what restriction enzymes will be used for the Restriction Digestion of Lamba DNA lab?
2. What do restriction enzymes do? Be specific. 1. At what voltage did we run our gel at? For how long?
2. List any observations you made when electrophoresis was started. |
Mrs. HusselsteinBiotechnology II Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom. Archives
December 2018
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