Bacteria in a miniprep are lysed using a buffer that contains EDTA. Why is the EDTA important?
What is a miniprep?
Bacteria in a miniprep are lysed using a buffer that contains EDTA. Why is the EDTA important?
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1. What is the function of arabinose in the LB/amp/ara agar plates
2. Predict the phenotype of a non-transformed bacteria under UV-light when growing on LB/amp agar plates and LB agar plates. 1. How is a bacterium that's been transformed with a plasmid usually selected?
2. What is the most common technique used to transform bacteria? Analyze the bacterial growth curve shown above. Identify the phase each statement describes.
1. The phase where bacteria are maturing and not dividing. 2. Growth rate and death rate are equal (no growth) due to limiting factors 3. This phase shows exponential growth. 4. Bacteria die due to lack of nutrients to inadequate environmental conditions. 1. What feature do you think a plasmid must contain in order to copy itself in a cell?
2. In order for a gene to be expressed what must be present? 1. What has to happen in order for the GFP to be expressed (assuming it is turned on)?
2. True or False. The plasmid glows when the the GFP gene is turned on. 1. Would you consider yourself a germophobe?
2. What might you be doing at home, school, or work that is propagating antibiotic resistance in bacteria? 1. What causes GFR to fluoresce under UV light?
2. What is the role of calcium chloride in a transformation? 1. What does the araC gene do in a pGLO transformation lab?
2. What does the bla gene do in a pGLO transformation lab? |
Mrs. HusselsteinBiotechnology II Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom. Archives
December 2018
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