2. Why do you think you needed to add more buffer after the protein mixture was loaded onto the column ?
1. Would vitamin B12 (1350 daltons) be fractionated by the column or excluded from the column?
2. Why do you think you needed to add more buffer after the protein mixture was loaded onto the column ?
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1. Define exclusion limit. What is it for our beads?
2. How will you determine which molecule has eluted? 1. How does size exclusion chromatography work?
2. What two molecules will we be analyzing using this technique? 1. Describe the shape of a primary protein structure.
2. Describe the shape of a tertiary protein structure. 1. According to your reading, what is one technical challenge in producing ethanol as a biofule?
2. What is the isoelectric point of a protein? |
Mrs. HusselsteinBiotechnology II Warm-Up questions. Please write your answers in the Warm-Up document provided weekly on Google Classroom. Archives
December 2018
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